Hydrophilic interaction chromatography analysis of biological compounds

MAKOTO TSUNODA, University of Tokyo, Tokyo, Japan (MAKOTOT@MOL.F.U-TOKYO.AC.JP)

HPLC is the most common approach to solve multiple analytical problems, because HPLC-based techniques allow for the separation of quite complicated mixtures of analytes with different physico-chemical properties. Reversed-phase liquid chromatography (RPLC) is the most popular LC separation mode. However, retention of hydrophilic compounds cannot be achieved on RPLC. This problem has been overcome by hydrophilic interaction chromatography (HILIC), which has rapidly gained popularity in recent years. HILIC can be used for a wide variety of hydrophilic compounds including biological compounds and drugs in biological fluids, pharmaceuticals, and foods. In my talk, I will describe analytical methods for biological compounds based on HPLC-fluorescence detection under HILIC conditions. Low-molecular-weight biothiols like cysteine, homocysteine, and glutathione and catechol compounds like norepinephrine and dopamine are studied as biological compounds. Application to human plasma or urine samples is also described.

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Abstract Reference & Short Personal Biography of Presenting Author

Makoto Tsunoda obtained a PhD in Graduate School of Pharmaceutical Sciences, University of Tokyo in Tokyo, Japan. After graduation, he worked as a postdoctoral research fellow at Ames Laboratory, Iowa, USA. He is currently associate professor at University of Tokyo where his research interest is in on-chip liquid chromatographic separation technique and development of bioanalytical methods for biological compounds. He has over 80 peer-reviewed papers, and 3 book chapters.