Automated Hydrolysis and Sample Preparation for the Analysis of 12 Opiates in Urine using the Thomson eXtreme Filter Vials® by LC-MS/MS

Nadine Koenig, Health Network Laboratories, USA
Crystal Xander, Health Network Laboratories, USA
Melanie Stauffer, Health Network Laboratories, USA
Dean Fritch, Analytical Associates, USA
Sam Ellis, Thomson Instrument Company, USA
Lisa Wanders, Thomson Instrument Company, USA
Yair Shachar, Yair Technologies, Israel

The use of hydrolysis in the analysis of opiates in urine has become standard practice in drug testing labs. Many laboratories currently use solid phase extraction or solid liquid extraction techniques in the sample preparation of urine for the analysis for opiates. This improved automated sample preparation method evaluates the robustness for the quantitative measurement of opiates in urine without the need for SPE/SLE. Thomson eXtreme Filter Vials provide a simple and efficient extraction technique that has demonstrated adequate analyte recovery, reduced matrix interferences and the elimination of solvent waste and other consumables. This validated method alleviates the need for sample clean-up by SPE thereby reducing the amount of equipment required, solvent usage and sample preparation time.