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Pre Conference Event

Biomolecules2


In this course, you will learn how to develop methods and implement CE for the separation of biomolecules. The course begins with an extensive review describing the factors that influence the electrophoretic migration of molecules in fluid solution. A phenomenological approach is employed that enables the student to take home a thorough understanding of electrophoretic processes. The course is designed for analytical chemists and analytical biochemists and those in related fields who wish to implement capillary electrophoresis in their laboratories. While appropriate for novice users, the course will especially benefit experienced users who wish to broaden their background. 
 
Who should attend
Chemists and biochemists interested in the separation of biomolecules, primarily proteins which are active pharmaceutical ingredients
   
 
Benefits to the Participants

  • Develop an understanding of fundamental electrophoretic processes
  • Learn the importance of buffer design and optimization
  • Selecting the modes of electrophoresis: CZE, CIEF and CSE

 

Issues to be covered

Introduction to Capillary Electrophoresis

  • The transition from slab-gels to capillaries
  • Using diagrams to help understand the impact of ion migration on separations
  • How to optimize resolution
  • Buffers and buffer additives
  • Commercial instrumentation

 

Capillary Zone Electrophoresis of Biomolecules

  • How to develop a well controlled separation
  • Case study, heparin

 

Capillary Isoelectric Focusing

  • How to select instruments, capillaries and ampholytes
  • Case study, erythropoetin

 

Capillary Sieving Electrophoresis

  • Introduction to polymer networks
  • Method development strategies
  • Size separations for proteins and DNA
  • Case study, monoclonal antibodies

 

Injection

  • When to use and how to optimize both electrokinetic and hydrodynamic injection

 

Stacking and trace enrichment

  • How to optimize stacking to solve sensitivity problems

 

Detection

  • Why corrected peak areas are required with CE
  • Types of detection including mass spectrometry (MS)
  • Case study, carbohydrate separations with detection by laser induced fluorescence and MS

 

Troubleshooting guide

  • Common and unusual problems are described

 


About The Lecturer
RobertDr. Robert Weinberger
Since 1991, Dr. Weinberger has been a consultant specializing in the field of High Performance Capillary Electrophoresis and related technologies. He is President of CE Technologies, Inc., a consulting firm specializing in contract research in HPCE. He taught the American Chemical Society Short Course entitled "Capillary Electrophoresis of Biomolecules" for 25 years and is the author of the textbook “Practical Capillary Electrophoresis”.
He has written some 100 Magazine articles for American Laboratory and is the author of over 50 technical publications, book chapters and patents in the fields of electrophoresis and liquid chromatography. He served as an expert witness in capillary electrophoresis patent litigation and provided expert opinions concerning the purity of potentially contaminated heparin samples. Food and drug contamination, dietary supplements and glycoprotein separations are recent areas of investigation.