Chirality at the dried blood spot:
Enantioselective analysis of pharmaceuticals on sub two micron columns

Ashraf Ghanem, University of Canberra, Australia

The pathology blood testing in the majority of commercial labs involves a whole venous blood sample in a tube from which the serum or plasma portions are separated, and run on an immunochemistry or biochemistry analyser. Until today, these testings are very efficient, however, the assays are generally expensive and not sensitive. Furthremore, the current technology for testing very much restricts a direct-to-consumer approach for providing test information because it requires the professional taking of blood by a skilled nurse. Dried blood spot has been introduced especialy in the doping in sports arena. This method is designed to detect and deter the use of substances and methods on the World Anti-Doping Agency (WADA) prohibited list. The WADA list prohibits all stimulants in competition and names about 60 examples. The most notorious is the chiral amphetamine, which stimulates the central nervous system by releasing neurotransmitters, such as dopamine, into the synapse between neurons and consequently combat fatigue. Many other substances including chiral β2 agonists and chiral β-blockers. In this presentation, we investigate the use of two recently commercialized sub-2 µm columns Chiralpak IG-U® and Chiralpak ID-U® for the fast HPLC enantioseparation of a set of drugs extracted form a dried blood spot.

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Abstract Reference & Short Personal Biography of Presenting Author

Ashraf Ghanem (University of Canberra) was honored with a Citation of the RACI for his contributions to the chemistry profession. Ghanem
studied at Helwan University, Cairo and the University of Stuttgart, and worked with Volker Schurig at the University of Tbingen for his PhD
(completed in 2002). He subsequently carried out postdoctoral research with Paul Mller at the University of Geneva (2002–2004), and was Head of the Biomedicinal Chemistry Unit at King Faisil Specialist Hospital and Research Centre, Riyadh (2004–2008). From 2008–2010, he was a research fellow and lecturer with Paul Haddad at the University of Tasmania, and after a visiting professorship with Nobuo Tanaka at the Kyoto Institute of Technology, he joined the faculty at the University of Canberra, where he was made professor in 2012. Ghanems research interests lie in the area of asymmetric and enantioselective catalysis and analysis. He has published a Microreview in the European Journal of Organic Chemistry on rhodium- catalyzed enantioselective CH functionalization reactions.

References
D. Ibrahim, A. Ghanem, Molecules 2019, 24 (7), 1287