Nanofibers as a modern extraction sorbent for drugs in biological matrices


Hedvika Raabová, Department of Analytical Chemistry, The Charles University, Faculty of Pharmacy in Hradec Králové, Hradec Králové, Czech Republic (raabovah@faf.cuni.cz)
Martina Háková, Department Of Analytical Chemistry, The Charles University, Faculty Of Pharmacy In Hradec Králové, Hradec Králové, Czech Republic
Jiří Chvojka, Department Of Nonwovens And Nanofibrous Materials, The Technical University Of Liberec, Faculty Of Textile Engineering, Liberec, Czech Republic
Jakub Erben, Department Of Nonwovens And Nanofibrous Materials, The Technical University Of Liberec, Faculty Of Textile Engineering, Liberec, Czech Republic
Lucie Chocholoušová Havlíková, Department Of Analytical Chemistry, The Charles University, Faculty Of Pharmacy In Hradec Králové, Hradec Králové, Czech Republic
Dalibor Šatínský, Department Of Analytical Chemistry, The Charles University, Faculty Of Pharmacy In Hradec Králové, Hradec Králové, Czech Republic

The laborious and time-consuming sample pretreatment is often required before the biological sample analysis. Restricted access materials are special type of sorbent primarily used for extraction of proteinaceous matrices. The matrix is purified from macromolecules and contained analytes are retained on sorbent at the same time. This ability of RAM is given by two types of surfaces with different hydrophilic and hydrophobic properties as well as by the porous structure of these sorbents. Placing these extraction columns into the column-switching chromatography system simplifies the analysis of the biological samples. 

The nanofibers have large surface area that is able to capture the analytes. Moreover, the structure of nanofibrous materials with narrow inter-fiber spaces may exclude the macromolecules via size exclusion mechanism. Based on these properties, it was assumed that the polycaprolactone nanofibers could work as RAM. Therefore, they were tested for this purpose, although the exact mechanism of protein removal and analyte retention has not yet been clarified.

The analytes were extracted from two different matrices to prove protein removing ability of polycaprolactone nanofibers. The methods for determination of b-lactam antibiotics in bovine milk and non-steroidal anti-inflammatory drugs in human serum were developed for this purpose. Both of the matrices were injected on extraction column manually filled with nanofibers and afterwards directly separated on analytical column in column-switching chromatography system. The nanofibers provided sufficient extraction efficiency for both group of analytes. Additionally, the nanofibrous sorbent was able to remove proteins to a sufficient extent to prevent column clogging and worsening of analyte detection.

The study was supported by specific research, no. SVV 260 412, GAČR project no. 17-08738S and GAUK project no. 766 218.


Abstract Reference & Short Personal Biography of Presenting Author

Hedvika Raabová (25) comes from Czech Republic. Currently, she is the PhD student at the Charles University, Faculty of Pharmacy in Hradec Králové, Department of Analytical Chemistry, where she is focused on pharmaceutical analysis. She received her Master of Pharmacy degree at the same faculty two years ago. The object of her research is using of nanofibrous polymers for extraction of drugs from biological samples. She uses the HPLC systems for this purpose. She spent part of her studies at the internship in Switzerland, where she worked in the research group of prof. Peter C. Hauser at the University of Basel. She gained experience with capillary electrophoresis and capacitive deionization on this stay.

Besides science and gaining new skills and knowledge, she is interested in travelling, sports and art. She speaks three languages – English, German and French, and would like to learn Spanish and Russian as well. In the future, she would like to work in the pharmaceutical research and development of the new drugs. 

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