2D-LC as an Automated Desalting Tool for MSD Analysis and as a Pharmaceutical Impurity Analysis

Xavier Maes, Agilent Technologies, Palma de Mallorca, Spain (xavier.maes@agilent.com)

The characterization and impurity analysis of therapeutic peptides and proteins requires the use of chromatographic separation combined with mass selective detection. Chromatographic separation methods for peptides and proteins, however, often use MS-incompatible mobile phases. This first part of the presentation demonstrates the use of multiple heart-cutting (MHC) two-dimensional liquid chromatography (2D-LC) as an automated desalting tool for mass selective detection). The peptide glucagon was analyzed in accordance with USP 39 in the first dimension, using an MS-incompatible mobile phase. Automated desalting and mass selective detection were then performed in the second dimension.

In the second part of the presentation, HiRes 2D-LC is used to achieve the separation of two closely eluting compounds, one of which is present at very low concentration, and is hidden under the other more highly concentrated compound peak. Chlorodifluorobenzoic acids and deamidated insulin were analyzed as standard substances and a real sample, respectively.

Short Biography of Presenting Author

Xavier Maes works at Agilent Technologies for 10 years. After being an LCMS application engineer, he is now a product specialist for liquid phase separation products. Xavier holds three masters, one in organic chemistry from the university of Paris 11, one in environmental science from the university of Coventry and the last one in Chemical Technology from IQS Barcelona.

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