Simplified Sample Prep of Antibodies for Purity Determination by SEC-HPLC Using the Thomson Filter Vials

Sam Ellis, Thomson Instrument Company, USA

The aggregation of protein therapeutics has become a major concern for the pharmaceutical industry and regulatory agencies. Protein aggregates can cause an adverse immune response and are typically monitored throughout the formulation and production of bio-therapeutics. Monitoring aggregates helps to minimize risks from therapeutic proteins in clinical applications by optimizing early formulations to reduce aggregation during production, storage, and shipping. Routine analysis of samples containing cell culture media and/or supernatant by HPLC can lead to performance issues. Here, we demonstrate using Thomson Filter Vials for sample preparation allows for small sample size, has minimal impact on preparation time, no detectable loss in product, and improved column performance leading to extended column lifetimes.