Achieving Improved Resolution and Sensitivity for Challenging Combinations of Separation Conditions in two-dimensional liquid chromatography.

Xavier Maes, International Distributor Organization, Agilent Technologies, Las Maravillas, Spain (

A major challenge during method development in two-dimensional liquid chromatography (2D-LC) can be the incompatibility between the first (1D) and second dimension (2D) solvent systems. For challenging combinations of separation systems, volume overload effects such as breakthrough or broad and distorted peaks in the 2D can be observed. Active solvent modulation (ASM) provides a valve-based approach to address this problem, leading to improved resolution and sensitivity in the 2D. This presentation explains the principle of ASM, and demonstrates its effect in the combination of two reversed-phase (RP) LC separations and a combination of hydrophilic interaction liquid chromatography (HILIC) and RPLC.

Short Biography of Presenting Author

Xavier Maes works at Agilent Technologies for 12 years. After being an LCMS application engineer, he is now a product specialist for liquid phase separation products. Xavier holds a master in organic chemistry from the university of Paris 11 and one in environmental science from the university of Coventry.

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