CESI-MS - a powerful selective and sensitive analytical tool for bottom-up and top-down proteomics

Amir Pri-Or, G-INCPM, Weizmann institute, Rehovot, Israel (amir.prior@weizmann.ac.il)
David Morgenstern, G-incpm, Weizmann Institute, Rehovot, Israel
Alexandra Gabashvili, G-incpm, Weizmann Institute, Rehovot, Israel
Dalia Elinger, G-incpm, Weizmann Institute, Rehovot, Israel
Yishai Levin, G-incpm, Weizmann Institute, Rehovot, Israel

Capillary electrophoresis (CE) is a microscale analytical technique, taking place in a single tube, open capillary. Separation using CE is based on the electrokinetic mobility differences of the analytes, dictated by their charge and size, and often is characterized by high peak efficiency and resolution. Hyphenation of CE to mass spectrometry (MS) yields a powerful analytical platform enabling the unambiguous identification of the separated peptides and proteins for both bottom-up and top-down proteomics approaches. CESI-MS is an orthogonal, unique and powerful analytical approach to RPLC-MS, as both methods complement each other in perfect manner. We present preliminary CESI-MS analysis of proteins and peptides from simple and complex mixtures and highlight the strengths and weaknesses of the platform.

 

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Short Biography of Presenting Author

Working in the analytical department of a pharmaceutical company and finishing his PhD in BioAnalytical Chemistry at the Vrije Universiteit (VU) Amsterdam, Amir is an experienced analytical researcher, focusing on separation techniques. Amir’s main expertise is capillary electrophoresis (CE) for analysis of small and large molecules, using diverse detection techniques, such as wave-resolved fluorescence (wrFlu) and state-of-the-art mass spectrometries (MS). Recently Amir joined the de Botton Institute for Protein Profiling team at the Nancy and Stephen Grand Israel National Center for Personalised Medicine (G-INCPM), Weizmann institute, where protein and peptide analysis is being performed using cutting edge instrumentation and techniques.