Multi-residue determination of 21 organic compounds in fish tissue using liquid chromatography-tandem mass spectrometry.

Frieda Schwartsburd, Kimron Veterinary Institute , Ministry of Agriculture, Rishon Lezion, Israel (friedasc@moag.gov.il)
Inbal Zaibel, The J. Blaustein Institutes For Desert Research, Ben Gurion University, Midreshet Ben Gurion, Israel
Gal Dagan, The J. Blaustein Institutes For Desert Research, Ben Gurion University, Midreshet Ben Gurion, Isarel
Dina Zilberg, The J. Blaustein Institutes For Desert Research, Ben Gurion University, Midreshet Ben Gurion, Israel
Malka Britzi, Kimron Veterinary Institute , Ministry Of Agriculture, Rishon Lezion, Israel

A rapid and sensitive multi-residue method for the determination of 21 organic contaminants belonging to different classes in fish tissue was developed and validated. The method involved a simple extraction step with acetonitrile followed by a cleanup step based on the QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) approach. Analysis was performed in a single run by liquid chromatography coupled with electrospray ionization and tandem mass spectrometry (LC-ESI-MS/MS). The accuracy values for all of the analytes were between 80 and 117%; relative standard deviation values were in the range of 1-18 %. Limits of quantitation of the target compounds varied from 0.02 to 5ng/gr.