Understanding New Porous Graphitic Carbon Chromatography Columns: Retention Mechanisms and Applications

Egidijus Machtejevas, Science and Lab Solutions, Merck, Darmstadt, Germany (egidijus.machtejevas@merckgroup.com)
Cory Muraco, Sigma-aldrich Corp., Bellefonte, Usa

Porous graphitic carbon (PGC) has been used for multiple applications in liquid chromatographic separations since the 1970's.  Although its retention mechanisms are yet to be elucidated, it is clear that PGC has unique retentive properties towards polar compounds. Recent advances in synthetic procedures have led to the development of a new PGC particle that displays somewhat different retentive properties than what has been investigated so far in the past.  This lecture will focus on fundamental investigations of polar retention effects using this new PGC particle.  After a brief review of fundamental studies that have been done in the past with PGC particles, data showcasing the fundamental differences between the two PGC particles now commercially available will be discussed to elucidate how analytes interact with the newly designed particles. In addition, application data involving difficulty to retain compounds will be shared highlighting how the theoretical studies can aid application and method development.  Finally, PGC is a unique stationary phase amongst more conventional HPLC stationary phases and further advancements in PGC particle design may result in even better resolving power for a wider range of compounds including effective separation of nitrosamine compounds found in pharmaceuticals.

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Short Biography of Presenting Author

Egidijus Machtejevas was born in Kaunas, Lithuania where he studied chemistry, biotechnology and separation sciences (1991-2001) at Kaunas University of Technology, Lithuania. After gaining his PhD in analytical chemistry 2001-2007 he worked as a post-doc with Prof Klaus Unger at Mainz University, Germany. Also two years spent at AstraZeneca (Sweden) where he set up and validated several various multidimensional systems for peptidomic analysis of different biological fluids. He joined the R&D Department at Merck KGaA in Darmstadt in 2008, in 2009 took marketing manager position for, 2010 - 2018 product manager for chromatography. Currently he is a Senior Technical Advisor in Analytical Chromatography Workflows group. Egidijus Machtejevas has twenty-five scientific papers and thirteen book chapters to his name, and his major focus areas include chromatography, multidimensional liquid chromatography, proteomics and mass spectrometry.