THE USING OF CHROMATOGRAPHIC METHODS FOR THE ANALYSIS OF POLYENE MACROLIDE ANTIBIOTICS IN ORDER TO DEVELOP INNOVATIVE ENVIRONMENTALLY FRIENDLY BIOTECHNOLOGICAL PROCESSES FOR THEIR PRODUCTION

Valery Belakhov, Schulich Faculty of Chemistry, Technion - Israel Institute of Technology, Haifa, Israel (chvalery@technion.ac.il)

Polyene macrolide antibiotics (PMA) constitute a large group of natural compounds, which includes more than 200 drug preparations that are active against yeast, yeast-like and filamentous fungi, both saprophytic and pathogenic species.

A specific feature of PMA is that they are mixtures of substances with similar physical and chemical properties. This can be an antibiotic complex of components of one subgroup of PMA, or a complex of components belonging to different subgroups of PMA, differing from each other in medical and biological properties. In this regard, the urgent problem is studying the component composition of both experimental and commercial batches of PMA with the aim of standardizing them, selecting highly selective strains, and using regulated biotechnological environmentally friendly methods to obtain drugs with the most favorable component composition.

The poster presentation systematizes the literature data concerning the use of various chromatographic methods for studying PMA, such as paper chromatography, thin layer chromatography, countercurrent distribution, column chromatography, high-performance liquid chromatography. Particular attention is paid to the study of the component composition of PMA.

The results of chromatographic analysis of PMA (primarily their components) are discussed, which lay the foundation for the development of more effective biotechnological methods for the production of PMA. The environmental aspects of the development and use of innovative methods for microbiological synthesis of PMA with a high content of the main highly effective component with the best medical and biological properties are considered.