Seeing the Unseen: Complete Analytical Control of Hidden Isomers in GLP-1 Peptides

Alexandra Kompaniets, Analytical R&D, TAPI, Netanya, Israel (alexandra.kompaniets@tapi.com)

Controlling isomers in complex peptides such as GLP-1 analogs poses a significant analytical challenge. Conventional single-method approaches often fail to separate or quantify all isomeric variants. Development of  an orthogonal analytical strategy combining UPLC, high-resolution LC-MS, and an enzymatic cleavage method that allows peptide fragmentation and targeted detection of isomers. This approach enables complete control and characterization of all isomeric species. The developed workflow ensures analytical robustness, product consistency, and regulatory confidence for complex peptide-based API.

Short Biography of Presenting Author

I'm an analytical R&D manager with more than 10 years exprience in small molecules, polymers, oligonucleotides and peptides API analyitcal development in TAPI.