Next Generation Blood Sampling For Mass Spectrometry Based Point-Of-Need Diagnostics

Dr. Fred E. Regnier

Distinguished Professor of Chemistry at Purdue University

Dr. Fred E. Regnier is a Distinguished Professor of Chemistry at Purdue University. He is generally regarded as one of the world authorities on proteomics. Additionally, he is an accomplished entrepreneur, cofounding several companies such as BioSeparations, PerSeptive Biosystems, Beyond Genomics, Novilytic, and Perfinity Biosystems. He also serves on the Boards of several companies. He has published more than 300 journal articles and 70 book chapters and reviews, has more than 40 patents, has edited two books, and has won numerous national and international awards and distinctions for his research in analytical chemistry and biochemistry. Dr. Regnier received his B.S. from the Nebraska State College and holds a Ph.D. from Oklahoma State University. He did his postdoctoral training at the University of Chicago and Harvard University under the direction of John H. Law and Edward O. Wilson, respectively.

A. Personal Statement
My research interests include developing integrated analytical systems for the analysis and characterization of complex protein mixtures using multidimensional separation systems and mass spectrometry. I have a long-standing interest in affinity chromatography and in developing novel chromatographic materials. I co-founded PerSeptive Biosystems, now part of Applied Biosystems, and developed the company’s POROS® resins. These materials are now widely used in purifying protein pharmaceuticals. The approach we propose in this project is a novel application of these technologies to produce stable protein drug formulations. I will provide expertise in peptide screening and in synthesizing novel derivatives.

B. Positions and Employment:
1968-1971        Assistant Professor - Biochemistry, Purdue University, West Lafayette, IN
1971-1976        Associate Professor - Biochemistry, Purdue University, West Lafayette, IN
1976-1990        Professor - Biochemistry, Purdue University, West Lafayette, IN
1990-2004        Professor - Chemistry, Purdue University, West Lafayette, IN
2004-present    Distinguished Professor of Chemistry, Purdue University, West Lafayette, IN

Awards and Honors:
1982     David B. Hime Award for Achievement in Chromatography.  Presented by the Chicago Chromatography Discussion Group.
1987    Stephen Dal Nogare Award for Achievements in Chromatography.  Presented by the Delaware Valley Chromatography Discussion Group..
1989    ACS Award in Chromatography.  Presented by the American Chemical Society,.
1993    Martin Gold Medal. Presented by the Chromatographic Society of Great Britian.  
1995    ISCO Award. Presented by Instrument Specialties Corporation.
1995    Pierce Award in Affinity Chromatography from the International.Society for Affinity Chromatography.
1996    Eastern Analytical Symposium Award for Achievements in Separation Science.  
           Distinguished Lecturer in the School of Science at the Univ. of Leiden,  Annual Scientific Awards
2001    Golay Award from the Dutch Chromatography Society.  
2002    The Chromatography award from the California Society for Separation Science (CaSSS). 

C. Selected peer-reviewed publications (from more than 400)
2012:

1. Effect of single amino acid substitution on oxidative modifications of the Parkinson’s disease-related protein, DJ-1. Madian, Ashraf G.; Hindupur, Jagadish; Hulleman, John D.; Diaz-Maldonado, Naomi; Mishra, Vartika R.; Guigard, Emmanuel; Kay, Cyril M.; Rochet, Jean-Christophe; Regnier, Fred E. (2012) Molecular and Cellular Proteomics (2012), 11(2), 010892/1-010892/15.

2011:

1. A lectin affinity workflow targeting glycosite-specific, cancer-related carbohydrate structures in trypsin-digested human plasma. Drake, Penelope M.; Schilling, Birgit; Niles, Richard K.; Braten, Miles; Johansen, Eric; Liu, Haichuan; Lerch, Michael; Sorensen, Dylan J.; Li, Bensheng; Allen, Simon; Hall, Steven C.; Witkowska, H. Ewa; Regnier, Fred E.; Gibson, Bradford W.; Fisher, Susan J. (2011) Analytical Chemistry, 408(1), 71-85.
2. Differential carbonylation of proteins as a function of in vivo oxidative stress. Madian, Ashraf G.; Myracle, Angela D.; Diaz-Maldonado, Naomi; Rochelle, Nishi S.; Janle, Elsa M.; Regnier, Fred E. (2011) Journal of Proteome Research, 10(9), 3959-3972.
3. Determining the effects of antioxidants on oxidative stress induced carbonylation of proteins. Madian, Ashraf G.; Myracle, Angela D.; Diaz-Maldonado, Naomi; Rochelle, Nishi S.; Janle, Elsa M.; Regnier, Fred E. (2011) Analytical Chemistry, 83(24), 9328-9336.

2010:

1. Proteomic Identification of Carbonylated Proteins and Their Oxidation Sites.  Madian, Ashraf G.; Regnier, Fred E.  Journal of Proteome Research (2010), 9(8), 3766-3780.
2. Coupling protein complex analysis to peptide based proteomics. Gao, Qiang; Madian, Ashraf G.; Liu, Xiuping; Adamec, Jiri; Regnier, Fred E. (2010) Journal of Chromatography, A 1217(49), 7661-7668.
3. Screening antibody and immunosorbent selectivity by two-dimensional liquid chromatography-MS/MS (2-D LC-MS/MS). Cho, Wonryeon; Jung, Kwanyoung; Regnier, Fred E.  (2010) Journal of Separation Science 33(10), 1438-1447.
4. Sweetening the pot: adding glycosylation to the biomarker discovery equation. Drake, Penelope M.; Cho, Wonryeon; Li, Bensheng; Prakobphol, Akraporn; Johansen, Eric; Anderson, N. Leigh; Regnier, Fred E.; Gibson, Bradford W.; Fisher, Susan J. (2010) Clinical Chemistry, 56(2), 223-236.
5. Protein-based multiplex assays: mock presubmissions to the US Food and Drug Administration. Regnier, Fred E.; Skates, Steven J.; Mesri, Mehdi; Rodriguez, Henry; Tezak, Zivana; Kondratovich, Marina V.; Alterman, Michail A.; Levin, Joshua D.; Roscoe, Donna; Reilly, Eugene; et al (2010) Clinical Chemistry, 56(2), 165-171.
6. In vitro stable isotope labeling for discovery of novel metabolites by liquid chromatography-mass spectrometry: Confirmation of γ-tocopherol metabolism in human A549 cell. Yang, Wen-Chu; Regnier, Fred E.; Jiang, Qing; Adamec, Jiri (2010) Journal of Chromatography, A 1217(5), 667-675.
7. Repeatability and reproducibility in proteomic identifications by liquid chromatography-tandem mass spectrometry. Tabb, David L.; Vega-Montoto, Lorenzo; Rudnick, Paul A.; Variyath, Asokan Mulayath; Ham, Amy-Joan L.; Bunk, David M.; Kilpatrick, Lisa E.; Billheimer, Dean D.; Blackman, Ronald K.; Cardasis, Helene L.; et al (2010) Journal of Proteome Research, 9(2), 761-776.

2009:

1. Intrinsic subtype-associated changes in the plasma proteome in breast cancer.  Nakshatri, Harikrishna; Qi, Guihong; You, Jinsam; Kerry, Bemis; Schneider, Bryan; Zon, Robin; Buck, Charles; Regnier, Fred; Wang, Mu.  Proteomics: Clinical Applications (2009), 3(11), 1305-1313.
2. Glycoproteomics of lectin based on narrow selectivity lectin affinity chromatography. Jung, Kwanyoung; Cho, Wonryeon; Regnier, Fred E. Journal of Proteome Research (2009), 8(2): 643-650.
3. Multi-site assessment of the precision and reproducibility of multiple reaction monitoring-based measurements of proteins in plasma. Addonna, Terri A. et al., Nature Biotechnology (2009), 27(7): 633-641.

2008:

1. Use of Glycan Targeting Antibodies To Identify Cancer-Associated Glycoproteins in Plasma of Breast Cancer Patients.  Cho, Wonryeon; Jung, Kwanyoung; Regnier, Fred E. Anal. Chem.   (2008), 80(14), 5286-5292.
2. Two-Dimensional Correlation Optimized Warping Algorithm for Aligning GCxGC-MS Data.   Zhang, Dabao; Huang, Xiaodong; Regnier, Fred E.; Zhang, Min.   Analytical Chemistry (Washington, DC, United States)  (2008),  80(8),  2664-2671.
3. Comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry peak sorting algorithm.    Oh, Cheolhwan; Huang, Xiaodong; Regnier, Fred E.; Buck, Charles; Zhang, Xiang.   Journal of Chromatography, A  (2008),  1179(2),  205-215.
4. Toward chromatographic analysis of interacting protein networks.    Liu, Xiuping; Yang, Wen-chu; Gao, Qiang; Regnier, Fred.   Journal of Chromatography, A  (2008),  1178(1-2),  24-32.
5. Differential Metabolomics Using Stable Isotope Labeling and Two-Dimensional Gas Chromatography with Time-of-Flight Mass Spectrometry.    Huang, Xiaodong; Regnier, Fred E.  Analytical Chemistry (Washington, DC, United States)  (2008),  80(1),  107-114.

2007:

1. Enhancement of the LC/MS Analysis of Fatty Acids through Derivatization and Stable Isotope Coding.   Yang, Wen-Chu; Adamec, Jiri; Regnier, Fred E.. Analytical Chemistry  (2007),  79(14),  5150-5157.  
2. An isotope coding strategy for proteomics involving both amine and carboxyl group labeling.    Regnier, Fred E.   Department of Chemistry and Cancer Center,  Purdue University,  West Lafayette,  IN,  USA.   Methods in Molecular Biology (Totowa, NJ, United States)  (2007),  359(Quantitative Proteomics by Mass Spectrometry),  125-133.
3. Destabilization of DJ-1 by Familial Substitution and Oxidative Modifications: Implications for Parkinson's Disease.  Hulleman, John D.; Mirzaei, Hamid; Guigard, Emmanuel; Taylor, Biochemistry  (2007),  46(19),  5776-5789
4. Identification of yeast oxidized proteins.  Mirzaei, Hamid; Regnier, Fred.    Journal of Chromatography, A  (2007),  1141(1),  22-31.   
5. Neural network prediction of peptide separation in strong anion exchange chromatography.   Oh, Cheolhwan; Zak, Stanislaw H.; Mirzaei, Hamid; Buck, Charles; Regnier, Fred E.; Zhang, Xiang.    Bioinformatics  (2007),  23(1),  114-118.  
6. A method for the identification of glycoproteins from human serum by a combination of lectin affinity chromatography along with anion exchange and Cu-IMAC selection of tryptic peptides.   Qiu, Ruiqing; Zhang, Xiang; Regnier, Fred E..    Journal of Chromatography, B: Analytical Technologies in the Biomedical and Life Sciences  (2007),  845(1),  143-150.  
7. Differential phase-contrast BioCD biosensor.     Zhao Ming; Cho Wonryeon; Regnier Fred; Nolte David    Applied optics  (2007),  46(24),  6196-209.
8. Adaptive interferometry of protein on a BioCD.     Peng Leilei; Varma Manoj M; Cho Wonryeon; Regnier Fred E; Nolte David D    Applied optics  (2007),  46(22),  5384-95.